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| Isolation and phosphate solubilizing capacity determination ofrhizosphere phosphate-solubilizing microorganism in King grass |
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| Abstract: |
| Phosphate-solubilizing microorganisms were isolated from King grass rhizospheres using Mengjinna organic
culture media. The phosphate solubilizing capacities were investigated from 41 strains based on phosphate-solubilizing
circle and molybdenum blue method. The results indicated that 21 strains had ability to solubilize organic phosphate on
Mengjinna organic culture medium and 48 h D/d (diameter of phosphate-dissolving zone/diameter of bacterial clone) value
was from 1.3to 7.0; 72 h D/d value was from 1.72 to 5.5; 120 h D/d value was from 1.33 to 4.67. In liquid culture, the
available phosphate increment was from 4.11 to 327.1 mg/mL using molybdenum blue method by inorganic phosphatesolubilizing
microorganism. Strains 2-6, 2-7-1, 2-9 and 4-4 had higher ability of organic phosphate dissolving, showed
great potential for agricultural use in microbiological fertilizer. |
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