| Effects on viability and morphology changes of melanoma B16F10 cells induced by stigmasterol and 茁-
sitosterol were studied by MTT, inverted phase microscope and fluorescence microscopy. The results indicated that 茁-
sitosterol (0.20, 0.30 mmol/L) inhibited the growth of B16F10 cells and significantly showed dose-dependence. Stigmasterol
(0.20, 0.25 mmol/L) also inhibited the growth of B16F10, but showed no dose-dependence. Meanwhile, significant apoptosis
was induced by stigmasterol and 茁-sitosterol. Identified by the increase of apoptotic bodies and death cells, and the
decrease of adherent cells, typical apoptosis appeared in B16F10 cells treated by stigmasterol for 48-72 h. A number of
cells in presence of 茁-sitosterol appeared vacuoles and some parts of cells appeared apoptotic bodies. Fluorescence
microscopy established that numbers of cells treated by stigmasterol and 茁-sitosterol appeared apoptotic characteristics,
like condensed chromosome, swollen nuclear and apoptotic body. Accordingly, stigmasterol and 茁-sitosterol partly inhibited
B16F10 cells growth through inducing the cells apoptosis. |
