| Microsatellite markers were used to distinguish Megalobrama terminalis, Parabramis pekinensis and Culter
recurviceps in Pearl River. Using genomic of M. terminalis, P. pekinensis and C. recurviceps as templates, PCR
amplifications were performed by using 108 pairs of primers which were designed based on Culterinae fishes microsatellite
markers selected from GenBank and literature, and 16 special microsatellite makers were selected through PAGE glue. The
result showed that, the maker F-524 could PCR an amplified DNA fragment between 309-320 bp in M. terminalis and C.
recurviceps; the maker L-4 could amplify an amplified DNA fragment between 160-201 bp in C. recurviceps and P.
pekinensis; the maker L -7 could amplify an amplified DNA fragment between 160 -190 bp in M. terminalis and P.
pekinensis, and it could also amplify an amplified DNA fragment between 123-143 bp in C. recurviceps. In early growth,
the morphological of M. terminalis, P. pekinensis and C. recurviceps were almost the same, thus, we couldn爷t identify them,
but when we used one specific marker alone or combined several specific markers together, we could quickly identify these
three species on molecular level |
