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| Reference gene selection for real-time quantitativePCR normalization under fusaric acid stress inBenincasa hispida Cogn. var. chieh-qua How |
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| Abstract: |
| Appropriate reference genes for specific experimental materials and conditions are critical for analyzing the
expression of target gene by real-time quantitative PCR. In this study, four common housekeeping genes GAPDH, Actin,
UBQ, CYP and two novel reference genes CACS, F-box were chosen for identifying the expression stability in Benincasa
hispida Cogn. var. chieh-qua How samples from various plant tissues (roots, stems and leaves) or under fusaric acid (FA)
stress. The expression stability of candidate genes was determined and ranked using RefFinder software. The results
revealed a remarkably stable expression pattern of Actin and F-box when samples were stressed by fusaric acid. F-box
gene showed the most stable expression level in different tissues. Additionally, expression of traditional housekeeping genes
GAPDH and CYP showed great variations and therefore they were considered unsuitable as reference genes. This study has
laid the foundation for the study of resistance-related genes expression for fusarium wilt in B. hispida Cogn. var. chieh-qua
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