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| AP2 transcription factor expression in cassava under droughtstress by real-time fluorescent quantitative PCR |
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| Abstract: |
| In order to study the expression patterns of two AP2 genes in cassava under drought stress, the cassava
variety SC5 was treated with drought stress and RNA was extracted from the leaf abscission zone of different time points.
We detected the different expression of AP2 transcription factors by SYBR Green玉real-time fluorescent quantitative PCR
with the cDNA obtained from reverse transcription as templates. The results showed that melting curves of both target genes
had only one peak, indicating that the primers were specific and the amplification efficiency of target genes were close to
reference gene Actin. The experimental results could be analyzed by 2-驻驻CT calculation method. After analysis, the result
showed that the two AP2 family members were induced under drought stress, the expression patterns were not identical and
there was certain correlation. These results were consistent with microarray hybridization results. |
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