| The complete coding sequence (registration number: KF939629.1) of carboxypeptidase (CP) gene screened
from cDNA library plasmid of Musca domestica was obtained by PCR conducted with the CP cNA library plasmid as
template. The basic physical and chemical properties of the gene and its coding protein, signal peptide and subcellular
localization were predicted and analyzed by the bioinformatics methods. Recombinant plasmid PEASY -E1 -CP was
constructed and induced expression in E. coli OrigamiB(DE3). The results showed that the CP-ORF was 1 284 bp in length
encoding 428 amino acids with a predicted molecular mass of 47.8 ku, pI of 6.10 and conserved domain of
carboxypeptidase family. The recombinant plasmid PEASY-E1-CP was induced by IPTG and the protein expressed in E.
coli |
