| The ant i f ungalmat er i alpr oduced by Pseudoal t er omonasr ut heni ca JI V-49 si gni f i cant l y i nhi bi t ed t he gr owt h
ofCandi da al bi cans,Cr ypt ococcusneof or man,Asper gi l l usf umi gat us,A.ni ger ,Tr i chopyht on r ubr um and T.ment agr ophyt es.
The ant i f ungalmat er i alwaspr el i mi nar i l y separ at ed f r om t he cr ude f er ment at i on br ot h ofJI V-49 by f i l t r at i on t hr ough 8 kDa
di al ysi s bag,decol or i zat i on wi t h char coal and pr eci pi t at i on wi t h acet one and et hanol . I t was f ur t her pur i f i ed by AB -8
macr opor ous r esi n absor pt i on chr omat ogr aphy,Sephadex G -100 and Sephadex G -25 gelf i l t r at i on chr omat ogr aphy.The
pur i f i ed ant i f ungalmat er i alshowed a si ngl e spoton si l i ca gelt hi n l ayerchr omat ogr aphy.The pur i f i ed ant i f ungalmat er i al
wasi dent i f i ed asa l i popept i de by ni nhydr i n r eact i on,UV s pect r um anal ysi sand i nf r ar ed spect r um anal ysi s.The i nhi bi t i on
t o f ungioft he pur i f i ed ant i f ungalmat er i alwas det ect ed.The r esul t s i ndi cat ed t hatt he ant i f ungalspect r um oft he pur i f i ed
ant i f ungalmat er i alf r om JI V-49 wasbr oadert han t hose oft he ant i f ungaldr ugscommonl y used i n cl i ni cal . |
