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| DNA f i ngerpri ntcons t ruct i on and genet i cdi vers i t y anal ys i sf orYuexuan l i nesi n Agr os t i ss t ol oni f er a L. |
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| Abstract: |
| Sequence-r el at ed ampl i f i ed pol ymor phi sm( SRAP)met hod wasused t o const r uctDNA f i nger pr i ntand det ect
t he genet i c di ver si t y of9 Yuexuan l i nesand 4 f or ei gn f i ne cul t i var sofAgr ost i sst ol oni f er a L.The r esul t si ndi cat ed t hat :( 1)
184 bands wer e gener at ed usi ng 17 outof32 pai r s pr i mer s,ofwhi ch 131 f r agment s wer e pol ymor phi c wi t h pol ymor phi c
bands( PPL)f or71% . ( 2) 13 l i neswer e di st i ngui shed f r om each ot herusi ng 4 pai rpr i mer s.The DNA f i nger pr i ntgr aphi c map
was const r uct ed wi t h 12 pol ymor phi c l ocii ncl uded. ( 3) The genet i c di st ance was 0. 0285-0. 7587 bet ween each ot heramong
13 l i nes. ( 4)Accor di ng t o t he UPGM A cl ust eranal ysi s,13 l i neswer e di vi ded i nt o 4 gr oupsatt he genet i c di st ance of0. 664.
These r esul t sai med t o pr ovi de f oundat i on f ori dent i f i cat i on and ext ensi on ofYuexuan l i nesi n A.s t ol oni f er a L. |
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