| :Ant hocyani ns ar e f l avonoi d der i vat i vesand have good ant i oxi dantcapaci t y.Br assi ca napus i s a ki nd ofoi l
pl antand wi despr ead al lover t he wor l d.However ,ant hocyani n l eveland ant i oxi t antcapaci t y ofB.napus ar e l ow.And,
l i t t l e at t ent i on i s pai d t o genet i c t r ansf or mat i on ofsnapdr agon Ant i r r hi num maj us ant hocyani n bi osynt hesi s t r anscr i pt i on
f act or s i nt o B.napus t o enhance t he ant hocyani n cont ent .I n t hi s st udy,Roseal 1 and Del i l a gene f r agmentwer e ampl i f i ed
by PCR f r om snapdr agon A.maj us cDNA,r espect i vel y.Then,t he t wo gene f r agment s wer e cl oned i nt o pDH51 i ncl udi ng
35S pr omot er and 35S t er mi nat or r espect i vel y.Fr agment s cont ai ni ng 35S pr omot er ,t ar getgene and 35S t er mi nat or wer e
l i gat ed i nt o pBI N19 r espect i vel y,r esul t i ng i n t he expr essi on vect orpBI N-Del i l a-Roseal 1 cont ai ni ng doubl e 35S pr omot er
and t er mi nat or .The cal l us oft he t r ansf or med B.napus t ur ned outt o be pur pl e compar ed t o t hatoft he wi l d-t ype st r ai n.
The r esul tpr ovi desbasi sf orusi ng genet i c engi neer i ng t echnol ogy t o i ncr ease t he ant hocyani n l evel sofB.napus. |
