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Genetic analysis and gene mapping of rice blastresistance in an indica rice Yuanbaozhan |
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Abstract: |
Rice blast, caused by fungus Magnaporthe grisea, is one of the most serious diseases of rice and has become
a serious threat to food security. Yuanbaozhan is a new rice material. Improving the rice resistance to rice blast through
genetic improvement methods is the most effective and safe way in the present. Yuanbaozhan has an broad -spectrum
resistance to rice blast pathogen, and it has high resistance to 23 strains among 25 blast strains collected from different
regions in Guangdong province. Its resistance frequency is 92%. In this study, we made a combination to establish genetic
population F1, F2 among which population is from the crosses between Yuanbaozhan and Yuexiangzhan. Evaluation was
conducted with Magnaprthe oryzae isolate GD00-193a, the results showed that the resistant and susceptible plants in F2
population fitted the ratio of 3:1, indicating that the resistance of Yuanbaozhan to the rice blast was controlled by a
dominant gene or a QTL locus. With the bulked-extrems and recessive-class approach analysis, the rice blast resistance
gene was mapped at the genome region between RM136 and RM549. |
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