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| Study on locus-specific DNA methylation polymorphismsamong donor cell lines and their associations withporcine somatic cell cloning efficiency |
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| Abstract: |
| Bisulfite restriction analysis (COBRA) combined with bisulfite sequencing (BS) were firstly carried out to
screen locus-specific DNA methylation markers in donor cell lines for improving porcine somatic cell cloning efficiency.
DNA methylation polymorphisms of eight differentially methylated regions (DRMs) among six donor cell lines and their
potential associations with porcine cloning achievements were analyzed. Result indicated that all DMRs presented relatively
high degree of DNA methylation polymorphisms, except for XIST -DMR2 and H19 -DMR3 with low extent of DNA
methylation variations below 10%. Moreover, three DMRs including LINE1-DMR1, POU5F1-DMR1, and NANOG-DMR1
presented the most DNA hypomethylated status in the most efficient cell line, and thus could be considered as potential
DNA methylation genetic markers for selecting optimum donor cell lines for porcine somatic cell cloning. |
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