| A liquid culture experiment was conducted to study the dynamic variations of the phosphate-solubilizingcapability and growth of the tested Bacillus subtilis HL-1 and medium pH within 168 h as well as the different effects ofinitial pH of culture media, carbon sources, nitrogen sources and the level of carbon concentration on phosphate -solubilizing capability of HL-1. The results indicated that a significant negative correlation was observed between the phosphate-solubilizing capability of HL-1 and the medium pH. The HL-1 displayed the best phosphate-solubilizincapability on condition that the initial pH of medium was 5.0, glucose with 10.0 g/L for source and ammoniumsulfate for nitrogen source. The soluble phosphorus content achieved 105.82 mg/L after the strain cultured for 168 h. The
test results reflect that the microbial phosphate-solubilizing capability can be influenced by many factors of the culture environment, the optimal combination of factors will not only be conducive to its effect under laboratory conditions, but also provide a theoretical basis for the development of microbial preparation process. |
