| Guava leaf water -extract was separated preliminarily using organic solvent extraction method. Four
components in petroleum ether phase (S), ethyl acetate phase (Y), n-butanol phase (Z) and water (W) were collected
separately. The challenge test results showed that there were no deaths observed after shrimp infected by white spot
syndrome virus (WSSV) incubated with the components in ethyl acetate phase (Y) or n-butanol phase (Z), respectively, but
the shrimp started to die after infected by WSSV incubated with the components in petroleum ether phase (S) 5 d, and all
died after 12 d. WSSV was incubated with guava leaf water-extract and its extraction content at different time and
quantified qPCR. The results showed that the number of
WSSV decreased with time passing whether in experimental
group or in control group, but still remained at a high level
at 120 h. The naked deoxyribonucleic acid (DNA) of WSSV
was incubated with guava leaf water -extract and four
extraction component respectively, no significantly
differences of WSSV gene number were observed comparing
to that at the experiment starting (P>0.05). |
