| Intramuscular (IM) preadipocytes were isolated from longissimus dorsi muscle obtained from 5-day old piglet of Bamei pigs by dtigestion of collagenase type II, and purified with differential adhesion method. The cells before and after differentiation were identified by RT -PCR and oil red O staining, responsively. The adherence rate and proliferation of the preadipocytes were detected, and variation in fat content in the cells induced differentiation process was determined by oil red O staining extraction, to study the growth characteristics and differentiation of the IM preadipocytes. The results showed that the IM preadipocytes isolated from Bamei pigs appeared to spindle-shape and fibroblast-like and well attached with 90% of maximum adherent rate. The cells grew well, and had S-type of growth curve which was in accord with normal growth pattern of cells. Though having poorly potential of spontaneous differentiation in vitro, cultured IM preadipocytes could differentiate into adipocytes with typical characteristics after adipogenic induced, and differentiation level significantly increased in time-dependent manner. |
