| Unigenes were downloaded from NCBI (http://www.ncbi.nlm.nih.gov/genbank/) to screen SSRs in completetranscriptome sequence of H. brasiliensis RRIM 600 (accession number：JT914190-JT981478). MicrosAtellite (MISA)software was used to identify and analyze the SSRs with 1-10 base repeat and bases above 12 bp. A total of 91 846 SSRswere found in H. brasiliensis RRIM 600, distributing in 42 859 unigenes, which accounted for 63.71% of the total numberof unigenes. The average length and distribution distance of the transcriptomic SSRs were about 9 bp and 1/0.92 kb,respectively. In the 91 846 SSRs, the hexamers were the main types with the SSR numbers of 52 615, accounting for 57.29% of all the SSRs. The potential of the transcriptomic SSRs for further usage was assessed. |
