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| cDNA cloning and bioinformatics of a ferritin heavy chain homologue from beetle, Monochamus alternatus |
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| Abstract: |
| Insect ferritin is composed of two subunits, which are similar to heavy and light chains of vertebrateferritins. A heavy chain homologue (HCH) cDNA of Monochamus alternatus, designated as Mafer (GenBank: KJ872588) wasobtained from a previously constructed DNA library. The open reading frame of Mafer is 669 bp in length, encoding 222amino acid residues with calculated molecular mass of 25.8 ku and isoelectric point of 5.72. Among the predicted Maferprotein sequence, a 18 amino acid signal peptide and cysteine domain were found, wich is in accord with the HCH ofvertebrates. But Mafer contains no iron responsive element (IRE) at its 5忆-UTR. The sequence alignment shows that Maferhas 93% and 69% identity with Anoplophora glabripennis HCH and Apriona germari HCH, respectively. Phylogenetic analysis indicats that M. alternatus and A. glabripennis was on the same branch, and M. alternatus is distantly related with
Drosophila melanogaster. |
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