| In order to reveal how different dyes in printing and dying wastewater influenced the microbial community in anaerobic sludge, eight kinds of dyes were used to domesticate the anaerobic decolorizing microbial community enriched
from the anaerobic sludge. The decolorizing abilities of eight different domesticated microbial communities were studied.
The DNA of eight domesticated microbial communities was extracted and amplified, and denaturing gradient gel
electrophoresis (DGGE) was applied to obtain the DNA fingerprint profile of those microbial communities. The results of the decolorizing experiment showed that the decolorizing rates of microbial communities domesticated by azo dyes were higher than those of microbial communities domesticated by anthraquinone dyes. The DNA fingerprint profile indicated that the structure of the enriched decolorizing microbial community changed after it was domesticated by different dyes. There were fewer bacterial species left after the enriched community was domesticated by Reactive Black 5 and Acid Blue 127. The
results of sequencing 12 dominant bands revealed that they respectively belonged to Lactobacillus, Enterococcus,
Lactococcus and Bifidobacterium and they could play important roles in the process of decolorization. |
