文章摘要
Screening and analysis of male sterile mutants derived from elite indica cultivar Huanghuazhan
  
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Author NameAffiliation
陈竹锋,卢嘉威,卢启清,王娜,王成旭,谢刚,周向阳,唐晓艳 深圳市分子设计育种重点实验室/深圳市作物分子设计育种研究院华南农业大学生命科学学院首都师范大学生命科学学院 
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Abstract:
      EMS (Ethylmethylsulfone) mutagenesis was used to generate a mutant library of elite indica caltivar Huanghuazhan. 317 independent male sterile mutants controlled by single receive genes were obtained. These mutants were roughly divided into 6 groups based on examination of floral organ morphology and pollen staining. Defects were found at EMS (Ethylmethylsulfone) mutagenesis was used to generate a mutant library of elite indica caltivar Huanghuazhan. 317 independent male sterile mutants controlled by single receive genes were obtained. These mutants were roughly divided into 6 groups based on examination of floral organ morphology and pollen staining. Defects were found at various developmental stages of pollen development in these mutants. These male sterile mutants lay the foundation for further study of molecular mechanisms governing male fertility and also provide ample materials for rice molecular design breeding. 98 no-pollen mutants were sequenced for TDR, an important gene for rice pollen development, which revealed3 different mutations in three mutant plants.
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