| A gas chromatographic method was used to determine volatile fatty acid, and the determination condition was optimized by orthogonal test in combination with single factor test. The results showed that the optimum detection parameters were as follow: sample volume of 1.5 μL, carrier gas flow rate of 75 mL/min, injection and detector temperatures at 220℃, 230℃ respectively, the column temperature conditions: temperature programmed (initial temperature of 100℃ for 2 min, with 6℃/min to 130℃ for 1 min, with 10℃/min to 190℃ for 2 min), by using FFAP capillary-column chromatography (30 m*0.32 mm*0.5 μm). Under these conditions, the volatile acid component could be completely separated in quickly and substantially symmetrical peak way. |
