| In order to explicit the genetic diversity of 38 strains Fusarium mangiferae from mango malformation
disease from Panzhihua, Sichuan, and Huaping, Yunnan in China, the ISSR technique was used. 72 bands were amplified
by 14 ISSR primers, with an average of 5.14 bands per primer. The size of the amplified products ranged from 300 bp to
2 000 bp. The genetic similarities among isolates ranged from 0.5972 to 0.9862. Isolates MG16 and MG17 were the most
similar(0.9862 genetic similarity), whereas isolates MG07 and MG33 were the least similar(0.5972) and were grouped in
separate clusters. At a genetic similarity of 0.76, the unweighted pair -group method with arithmetic mean analysis
separated the isolates into five distinct clusters, comprising 33, two, one, one and one isolate, respectively. The ISSR data
supported the conclusion that the isolates of F. mangiferae were not necessarily related by geographic origin, type of tissue
or the mango cultivar from which they were isolated. This is the first report on the diversity of F. mangiferae species
associated with mango malformation in China. |
