This study analyzed the SSRs in the genome of Sclerotinia sclerotiorum according to the genome sequence
data from public database. Totally we found 1 693 SSRs, most of them were 6 and 4 bases SSRs which appeared 577 and
449 times, respectively. 1 base, 3 bases and 5 bases SSRs appeared 97, 180 and 57 times, respectively. There were 340
SSRs from 308 putative gene sequences, 284 of them contained only 1 SSR, accounting for 92.21% of the total; 17 of genes
contained 2 SSRs, 6 genes contained 3 SSRs and only 1 gene contained 4 SSRs. Compared with SSRs from whole genome,
the SSRs with most frequency were 3 and 6 bases SSRs, which meant that under the selection pressure, the SSRs in gene
sequences were tend to be integer multiples of gene code number 3, this was consistent with the research on other species.
To analyze the functions of the genes contained more than 2 SSRs of S. sclerotiorum, we blast all these 24 genes by CCD
software from NCBI. Among them, only 6 genes contained conserved proteins domains and most of these domains were
related to transcription and DNA reparation. |