| In order to improve the biotransformation efficiency of culture medium, effects of components from
different culture media on biological characteristics and dynamic nutrient utilization of Volvariella volvacea were
studied by observing biological characteristics of stock culture, pre-culture spawn and spawn, extracellular enzyme
activities and consumption of nutrient components. The results showed that compared with other test groups, the
media with high content of Camellia oleifera shell and peanut hull were more suitable for straw mushroom cultivation,
in which the mycelium exhibited high extracellular enzyme activities and nutrient utilization. At the stage of fruiting
body formation, the cellulase activities in media with high content of C. oleifera shell and peanut hull were 3.36 U/g
and 3.23 U/g, respectively, the hemicellulase activities were 4.61 U/g and 4.87 U/g, respectively, the laccase
activities were 5.217 U/g and 4.23 U/g, respectively. During the cultivation of V. volvacea, the utilization of cellulose
increased by 8.79 and 7.07 times, respectively; the utilization of hemicellulose increased by 9.68 and 7.57 times,
respectively; the utilization of lignin increased by 18.55 and 13.59 times, respectively, and the biological
characteristics of stock spawn, pre-culture spawn and spawn were good compared with the control. While mycelium
cultivated on media with high content of bagasse and corn bran exhibited low extracellular enzyme activities, nutrient
utilization and poor biological characteristics, which indicated that these media were not suit for straw mushroom
cultivation. Therefore, it could be seen that components of culture media had significant influence on secretion and
activity of extracellular enzymes and nutrient utilization abilities of mycelium, and then the biological characteristics and quality of V. volvacea. |
