| The sterilized seeds of Ningqi 1of Lycium barbarum L. and Nongda 1of Lycopersicon esculentum Mill were germinated on MS or 1/2MS medium to acquire aseptic seedlings, and then, using the seedlings to induce callus on MS medium supplemented. A stable cell suspension culture system was established with callus of L. barbarum L. and L. esculentum Mill after several rounds of subculture.The results showed that supplement of 1.0 mg/L 2, 4-D and 1.5 mg/L KT for L. barbarum L. and 1.0 mg/L 2, 4-D and 1.0 mg/L KT for L. esculentum Mill in MS solid culture medium are the best condition for inducing callus both of them, and achieved 68.45% and 64.33% induction rate respectively. Treating suspension cell of 0.5% enzyme(Pectinase), 1% cellulase(Cellulase), isolated and R-10 0.2%(Macerozyme R-10), 0.2%, hemicellulase (Hemicellulase) at 30, pH 5.0-6.0 with a 12~16 h shaking resulted in the protoplast actvitity for 2 days. |
