The tolerance of a new yeast preparation, of which main component is active yeast, in a simulated
gastrointestinal tract model was analyzed. Firstly the yeasts growth characteristic experiment was conducted which
included growth curve in certain period of time, pH variation and coherency by the method of direct counting with
blood count board to analyze its growth characteristics, then determine yeasts dynamic testing under the acidic and
hypertonic environment in a simulated gastrointestinal tract model as well as its capacity at different temperature
conditions. Ultimately, it proved that the yeast could grow well under circumstances of the acidic environment at
pH2, concentration of bile salt of 1%, salt of 22%, and also at 64in a short time, moreover. In the range of
tolerance for probiotics, its higher survival rate and content could meet the best possible probiotic functional needs,
to provide theoretical basis for the clinical application and a large-scale production in the future. |