| The seeds of stylosanthes were irradiated by 60Co-ray. Then the implementation of individualselection to stylosanthes progeny continued for two years. The plant height, weight per plant, phenology and diseaserate of anthracnose were determined on M2 generation, and these data were analyzed by variance analysis. Six linesof M2 generation and two control varieties of stylosanthes were analyzed by SRAP analysis and cluster analysis. Theresults showed that the weight per plant of 10 lines of M2 generation were significantly greater than that of Nchp1(CK1). Compared with CK1, flowering stage of plant was earlier in 12 lines, plant height of 21 lines were greater,disease rate of 13 lines were less. SRAP analysis revealed that 528 effective bands were selected from 39 pairs ofprimers, each pair of primer amplified 13 bands, the average percentage of polymorphic bands was 91.67% andaverage PIC was 0.861. The Neis genetic similaritycoefficient of all treatments ranged from 0.485 to0.670, and the average Neis coefficient was 0.589.Using GS 0.5700 as a baseline, these 8 stylosantheslines were classified into two major groups by UPGMAcluster analysis, named group and group. Group included Reyan 2, group ncluded nchp1 and 6 derivative progeny of nchp1. Using GS 0.6250 as a baseline, group was divided into three small classes,nchpzh004 and nchpzh01101 were in the first class, nchpzh021 nchpzh01102 and nchp1 were put in thesecond class, nchpzh08 and nchpzh401 belonged to the third class. These results demonstrated that geneticvariation had diversity in M2 generation of irradiated stylosanthes both at agronomic traits and molecular level.There was significant detection rate in examining genetic polymorphism at genome level by using SRAPmolecular marker on stylosanthes. |
