| The banana MYB gene was cloned in silico based on the EST sequences from Unigene of NCBI.Some characters of MYB protein were analyzed and predicted by the tools of bioinformatics in the following aspects,including the composition of amino acid sequence, hydrophobicity or hydrophilicity, secondary and tertiary structureof protein and function. Results showed that the coding sequence of banana MYB was 1 266 bp, containing a 1 266bp ORF, which encoded 431 amino acids. As banana MYB contained one typical MYB domain, it could be concludedthat it belonged to R1-MYB subfamily. It was highly conservative compared with MYB from Triticum aestivum andother plant species in sequence composition, advanced structure and activity sites. The results could provide the basisfor MYB gene cloning in experiment and further research on its function. |
