| Strain LYM3, isolated from Rauvolfia vomitoria Afzel, had higher antagonistic effect against
Colletotrichum musae, and its antagonistic activity was 64% in plate. According to its morphological characteristics,
and 16S rDNA sequence analysis, the strain LYM3 was identified as Bacillus subtilis. Using single-factor method袁
the fermentation conditions of its antibacterial substance production were optimized. The optimal medium contained
2% soluble starch, 1% tryptone, 0.8% yeast extract, 1% NaCl, and initial pH was 6. After 60 h culture at 28益, 180
r/min, the antibacterial substance had higher yield, and the diameter of inhibition zone was up to 3.42 cm |
