| In a bid to product high -protein feed through double enzymatic degradation of cellulose and
microbial fermentation, the citrus peel residue was chosen as the raw material by the superfine grinding technology of
pretreatment. With the soluble protein and reducing sugar as examining index, the main impact factors including
adding amount of pectase and cellulose, system pH, yeast inoculation quantity and cultivate time were investigated.
The technological conditions of fermentation were optimized by orthogonal experiment. The results showed that the
best technological conditions of fermentation were as follows: 0.06 g/100g of pectase and 0.02 g/100g of cellulose at
50益in pH 5.0 for 1 h, the citrus peel residue with efficacy reducing sugar and yeast exploitation degree was
obtained. Then the citrus peel residue was cooled to 35益, inoculation quantity 10.0% (V/V), pH 4.0 and cultivate
time 5 d were optimal for the increasing of protein content in fermentation production, the yield of soluble protein
was 101.9 mg/L. |
