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Content differences of total polysaccharides,total alkaloidsand total flavonoids from stems and leaves of Dendrobiumcandidum at different growth ages |
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Abstract: |
To provide scientific basis for making full use of Dendrobium candidum resources,contentdifferences of total polysaccharides,total alkaloids and total flavonoids from stems and leaves of D. candidumat different growth ages were determined using phenol vitriolic method,acid-dye and Al(NO3)3 colorimetricmethod. Results showed that there were significant dif ferences in the contents of total polysaccharides,total alkaloids and total flavonoids from stems and leaves of D. candidum at dif ferent growth ages. Totalpolysaccharides mainly concentrated in stems,but total alkaloids and total flavonoids mainly concentratedin leaves. The highest content of total polysaccharides was about 35.32% for stem of two-year D. candidum,but the highest content of total alkaloids was about 0.036% for one-year leaf,and the highest content oftotal flavonoids was about 0.104% for half-year leaf. Content of total polysaccharides gradually decreasedwith growth age,and contents of total alkaloids and total f lavonoids of D. candidum in three years weresignificantly high. When the growth age reached four or five years,the contents of total alkaloids and totalf lavonoids were signif icantly low. Each stem andlea f of D. candidum contained dif f er ent activein g r ed ien t s,so b o t h o f t h em co u l d be used a smedicinal resources. In order to obtain the bestbenefit and considering the growth cycle,biomassand medicinal ingredients and so on,the appropriatetime for artificial-cultivation of D. candidum wasthree years and not more than 4 years. |
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