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| Reaction conditions optimization of cell wall degradingenzymes from mango bacterial leaf spot pathogen |
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| Abstract: |
| To optimize the reaction conditions of cell wall degrading enzymes from bacterial leaf spot pathogens ofMangifera indica Linn（mango），3，5-dinitrosalicylic acid method（DNS method）was used to determine the activitiesof cell wall degrading enzymes（CWDEs）with the induction substrate of 1% sodium carboxymethyl cellulose（CMC），0.5% CMC+0.5% pectin and 1% pectin，respectively；and these CWDEs assay conditions in terms of temperature，time，pH，ect were optimized. The results showed that，1.0% CMC was the most suitable substrate for the induction ofcarboxymethyl cellulose（Cx），whereas 1.0% pectin was most suitable for the induction of polygalacturonase（PG）andpectin methylgalactuionase（PMG）. The activities of Cx and PG were the highest at 60℃ and 50℃，respectively，for60 min and 20 min，respectively；the activities of Cx and PG were the highest when the pH value reached 4.6 and 6.6，respectively. |
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