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| Optimization of enrichment technology of total saponinsin leaves of Rosa davurica Pall. with macroporous resin |
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| Abstract: |
| To investigate the enrichment technology of total saponins（ TS） in leaves of Rosa davurica Pall.with macroporous resin，by both static and dynamic absorption-desorption experiments，the optimum purificationconditions with D-101 macroporous resin were determined using absorption and desorption ratio of TS asevaluating indicators. The results showed that the optinal enrichment canditions were as follows：the TS contentof the sample，adsorption rate，pH value of sample，content of ethanol eluant，desorption rate and volume ofeluant were one-fifth content of the raw extracts solution，2 BV/h，7 to 9，70%，2 BV/h，and 7 BV respectively.The average absorption and desorption rates were 81.33% and 86.31% under the optimum conditions. Afterpurification，the TS content in extracts was from 16.32% to 48.13%，which offered 3 times of enrichment withresins. The results indicated that purification of TS in leaves of R. davurica Pall. with D-101 macroporous resinwas effective. |
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