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| Effects of eyestalk ablation on brood-stock reproductionand environmental factors on larva survival rateand development time of Lysmata amboinensis |
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| Abstract: |
| This experiment used tweezers hot method to remove the unilateral eyestalk of parent shrimp，Lysmataamboinensis，studied the effects of unilateral eyestalk ablation on growth，amount of carrying eggs，hatching rate，numberof larvae，molt cycle and spawn cycle，and using manual controlled experiment tested the effects of different temperatures（20，23，26，29，32℃），different salinities（24，27，30，33，36）and different diets（chlorella，Chaetoceros，rotifer，artemia）on the survival rate and growth rate of L. amboinensis larvae. The results showed that eyestalk ablation hadsignificant impact on the growth rate of parent shrimp. During the test（126 d），the average lengths of parent shrimps ingroup 1（eyestalk ablation of one parent shrimp），group 2（eyestalk ablation of both parent shrimps）and control group（noeyestalk ablation）increased by 24.2（±2.3）mm，24.2（±2.7）mm and 16.2（±0.8）mm，respectively. The eyestalk ablationof parent shrimp had significant impact on the amount of carrying eggs，the amount of eggs of group 1，group 2 and controlgroup were 1 746.0（±1.5）ind/tail，1 982.0（±44.1）ind/tail and 1 343.0（±25.2）ind/tail，respectively. The numbers oflarvae of experiment groups were significantly higher than that of the control group，were 1 146.0（±5.5）ind/tail，1 254.0（±26.3）ind/tail and 699.3（±7.8）ind/tail，respectively. After eyestalk ablation，the molt times of parent shrimps increased，were 12，12，11 times，respectively；the spawn cycle shorted，were 10.8 d，10.0 d and 11 d，respectively. For larval growth，the optimum temperature was 23-29℃，the best temperature was 26℃；the optimum salinity was 27-33，the best livingsalinity was 33；the best diet for larval of Z1-Z2 waschaetoceros，for larval of Z3-Z6 was artemia. So when thelarval was Z1-Z2，we should feed priority to chaetoceroswith rotifer and artemia，when the larval was Z3-Z6，weshould feed priority to artemia with single-celled algae toimprove the diet effect. |
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