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| Construction of attenuated salmonella typhimuriumharboring DNA vaccine of FMDV andits safety and stability |
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| Abstract: |
| To explore the oral immunization feasibility of live-attenuated salmonella typhimurium for FMDVDNA vaccine，the O type FMDV VP1 gene was amplified by PCR and inserted into expression vector pIRES toconstruct pIRES-VP1. The expression of VP1 gene was proved by indirect immunofluorescence assay in BHK-21cell. The recombinant plasmid was transformed into the attenuated salmonella typhimurium 2266 by calciumchloride process. In order to research the stability and security of recombinant bacteria in mice，immuned miceof recombinant bacteria and strengthen the immune after 2 weeks at the dosage of 108，109，1010 CFU by oraladministration to study the stability of recombinant plasmid in vitro by recombinant bacteria in vitro batches. Theresults showed that 108，109 CFU dose immuned mice，the survival rate was 100% by 1010 CFU dose，clusterphenomenon occurred in mice，and mice became slow.Recombinant bacteria after 8 generation and immunemice in vitro isolated f rom the spleen and liveridentification showed that the recombinant bacteria2266（pIRES - VP1） both in vivo and in vitro hadgood stability. |
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