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| Cloning and sequence analysis of complete genome ofporcine circovirus isolated from Hainan province |
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| Abstract: |
| To understand the molecular characteristics of PCV2 isolated from Hainan province，the completegenome of PCV2 of some PMWS-suspected samples from Hainan province were amplified sequence analysis wascarried out. The results showed that a total of 8 PCV2 strains was isolated and identified，and all the isolates belongedto genotype 2b，of which 7 isolates were grouped into cluster PCV2b-1C and 1 isolate was grouped into cluster PCV2b-1A/1B. The ORF2 gene of 8 PCV2 strains were 705 bp，encoding 234 amino acids. The main antigen epitope areachanged significantly in Cap protein of PCV2. The identity of PCV2 ORF2 sequence among 8 isolates was 95.3%-99.7%of nucleotide similarity，and 93.6%-100% of deduced amino acid similarity. They had 91.0%-99.9% nucleotide similarityand 91.0%-99.6% deduced amino acid similarity with AY682994，AF381175，JX945577，JX682407 and AY180397；90.0%-97.0% nucleotide similarity and 88.0%-97.9% deduced amino acid similarity with NC_005148，JQ994268，KJ187306，AF201307，AF454546 and AY754020；98.2%-100.0% nucleotide similarity and 94.9%-100.0% deducedamino acid similarity with SH；90.6%-91.7% nucleotide similarity and 89.7%-91.0% deduced amino acid similarity withLG. This study got the basic epidemiological data for the prevention and control of PCV-2 in Hainan Province，it can alsohelp the choice of PCV-2 vaccine. |
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