文章摘要
Comparative study on indirect ELISA and neutralization testof O type FMDV vaccine of porcine serum antibody
  
DOI:
Author NameAffiliation
刘清源,向 华,黄 元,等 华南农业大学兽医学院广东省农科院动物卫生研究所/广东省兽医公共卫生公共实验室中国农业科学院兰州兽医研究所/家畜疫病病原微生物国家重点实验室 
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Abstract:
      The serum neutralizing antibody titer reflects the ability of serum neutralization on virus infection. It isan important index for vaccine evaluation. In this study,the O type FMDV of 120 serum samples collected from threedomestic large-scale pig farms were detected by ELISA and VNT, and the coincidence rates and positive detectionrate of the two methods were compared. The results showed that with the two methods,the positive coincidence ratesand negative coincidence rates were 77.3% and 72.2%,the total coincidence rate was 75%. Both of the methods couldeffectively detect FMD antibody level in serum of pigs. However,there was a certain proportion of false positive or falsenegative results by indirect ELISA. NT was more suitable for FMD serological monitoring and survey.
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