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| Extraction of antibacterial peptides from Musca domestica andtheir effects on disease resistance of Litopenaeus vannamei |
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| LI Lin1，2，SUN Yu-ping2，HUANG Yan-hua2，CHEN Bing2，CAO Jun-ming2，WANG Guo-xia2，LI Yong-juan2，MA Yong-ping1，2（1. College of Animal Science，South China Agricultural University，Guangzhou 510642，China；2. Institute of Animal Science，Guangdong Academy of Agricultural Sciences，Guangzhou 510640，China）Abstract：This experiment determined the extraction process of antibacterial peptides from Musca domesticaand evaluated the effects of antibacterial peptides of M. domestica on anti-white spot syndrome virus capacityin Litopenaeus vannamei. Maggots with good growth were screened out by ultrasonic induced method，and thenantibacterial peptides were extracted through tissue homogenate，ammonium acetate extraction，heat treatment，freezethawtreatment，salting out，dialysis，distillation and concentration，the concentration and yield were determined byspectrophotometry，the antibacterial activity was determined by turbidimetry，finally，shrimps were fed with fourexperimental diets that prepared by supplementing into the basal diet with 0 mg/kg，40 mg/kg，80 mg/kg and 120mg/kg antibacterial peptides of M. domestica. After 8-week feeding trial，shrimps were injected with WSSV and fed for52 hours. The results showed that，at the age of 4 days，the maggots bred by M. domestica were in good growth conditionsafter stimulation，and the yield was the highest. The concentration of antimicrobial peptides was 25.05 mg/mL，thepeptide extracting ratio was 2.36%. Diluted by 11 times，the inhibitory rate and antibacterial activity against Pseudomonasaeruginosa were 21.05% and 24.58 U. Compared with non-supplemented group，the accumulative mortality rates of 40mg/kg and 80 mg/kg groups were significantly lower. The relative percent survival rates of supplemented groups were42.42%，33.33% and 9.09%. It indicated that the extraction process could improve the extraction efficiency and ensurethe stability and antibacterial activity of antibacterialpeptides. The supplementation of antibacterial peptidesinto diets could improve the anti-white spot syndromevirus capacity in L. vannamei. |
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