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| Cloning and expression analysis of cinnamate 4-hydroxylasegene from Perilla frutescens |
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| Abstract: |
| In order to clarify the molecular mechanism of romarinic acid（RA）biosynthesis，the cDNA of cinnamate4-hydroxylase（PerC4H）gene was firstly cloned from the leaves of Perilla frutescens（GenBank accession No：KM434189）by homology-based cloning and rapid amplification of cDNA ends（RACE）technique. The full-length ofPerC4H cDNA was 1 667 bp，containing 1 518 bp open reading frame（ORF）which encoded a pepitide of 505 amanioacids and two non-coding regions，21 bp 5′UTR and 95 bp 3′UTR. Phylogenetic tree analysis showed that PerC4Hhad the closest relationship with Salvia miltiorrhiza and Scutellaria baicalensis. Real time quantitative PCR showedthat expression of PerC4H was the highest in root，moderate in stem，and the lowest in leaf. Further expression analysisshowed that Gibberellins and MeJA treatments could up-regulate the transcript level of PerC4H，while ABA and darktreatments could down-regulate its transcription level in some degree. |
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