| To evaluate the effects of antibacterial peptides of Musca domestica (ABPs-MD) on the resistance
ability to white spot syndrome virus (WSSV) in Litopenaeus vannamei, six experimental diets were prepared by
supplementing into the basal diet with 0, 1, 2, 3, 4 and 5 g/kg ABPs-MD, respectively. 960 juvenile L. vannamei
with an initial average weight of 0.86 (0.01) g were randomly divided to 6 groups. After 8-week feeding trial,
shrimps were injected with WSSV. The results showed that supplementing ABPs-MD at 3 g/kg significantly reduced
the cumulative mortality. The highest number of haemocytes and hemolymph phagotrophy rate were both obtained in
the shrimps fed the diet with ABPs-MD at 3 g/kg, significantly higher than those in the control, 1 g/kg and 5 g/kg
groups. When ABPs-MD was supplemented at 2-3 g/kg, the shrimps showed a higher level in activities of POD,
AKP, LZM and T-AOC in serum, significantly higher than those in the control group. The highest activities of SOD
in serum, AKP and T-AOC in hepatopancreas were all obtained in the shrimps fed the diet with ABPs-MD at 4 g/kg,
significantly higher than those in the control group. The highest activity of LZM was obtained in the shrimps fed the
diet with ABPs-MD at 3 g/kg, significantly higher than
that in the control, 1, 4 and 5 g/kg groups. The results
suggested that the dietary supplementation of
antibacterial peptides could improve the non-specific
immunity and disease resistance to WSSV in L.
vannamei. |
