|
| Optimization of extraction of paralytic shellfish poisoningfrom Alexandrium minimum by melting-freezing method |
| |
| DOI： |
|
| Hits: 1660 |
| Download times: 834 |
| Abstract: |
| To enhance the broken rate of Alexandrium minimum，to keep the composition unchanged and the
toxicity of Paralytic Shellfish Poisoning（PSP）in the extraction process，the freezing-melting extraction method for
PSP was used in this paper. Firstly，five single factor experiments were conducted to study the effects of pH，freezing
temperature，melting temperature，freezing time and melting times on broken rate and relative fluorescence value，
respectively. Then Box-Behnken response surface optimization was used to obtain the optimum conditions，with the
relative fluorescence value as response value. The results showed that，the optimum conditions for extraction of PSP
by melting-freezing method were as follows：pH 7.66，melting times of 3.45，at 36.99℃. Under these conditions，
the relative fluorescence value and virulence were 200.347，7 034 MU，respectively. In the verified experiment，
the conditions for PSP extraction from A. minimum
were：pH 7.8，melting times of 3，at 37℃，under
these conditions，the relative fluorescence value and
virulence were 188.923（±10.96），6 616（±101）MU，
respectively |
|
View Full Text
View/Add Comment Download reader |
