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| Fermentation medium optimization and enzyme propertiesof nitrite reductase produced by Bacillus sp. C |
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| Abstract: |
| The fermentation conditions of nitrite reductase produced by Bacillus sp. C were optimized by single
factor experiment and response surface analysis. The optimal fermentation conditions were as follows：2.26% glucose，
0.2% peptone，NH4Cl 0.12%，K2HPO4·H2O 0.2%，MgSO4 0.075%，KCl 0.1%，FeSO4 0.004%，NaNO2 0.2%. In
these conditions，the enzyme activity reached 317.82 U/mL，increased by 67.05% compared with the activity before
optimization with 190.26 U/mL. The results showed that the nitrite reductase was activated by Mn2+，Mg2+，Na+ and
inhibited by Fe2+ and EDTA；the optimal temperature was 30℃，the optimal pH was 7.4；the enzyme preserved higher
activity at the temperature below 50℃ and pH 7.0-8.0. Compared with the existing literature，the yield of nitrite
reductase produced by Bacillus sp. C was higher |
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