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| Detection of virulence genes in Aeromonas hydrophila and thepathogenicity of different genotype isolates to swordtail |
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| Abstract: |
| Four main extracellular virulence factors of Aeromonas hydrophila， including aerolysin（aer） ，
hemolysin（hlyA） ， extracellular protease（epa）and cytotoxic enterotoxin（act） ， were investigated in 60 isolates
of A. hydrophila by PCR. These 60 stains were isolated from diseased fishes from Jiangxi and Guangdong province
in 2008-2009. The majority（58.33%）of the strains carried the four virulence genes. aer + hlyA + epa + act + virulence
genotype isolates were major in A. hydrophila isolates . 12 representative strains from these genotypes were randomly
selected， and individually injected into swordtail. The results showed that the isolates with more virulence factors had
stronger pathogenicity to swordtail. aer + hlyA + epa + act + genotype isolates were more virulent to the swordtail compared to
others. |
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