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| Establishment of duplex PCR assay for identificationof pseudorabies virus |
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| Abstract: |
| Based on the gB and gE genes of pseudorabies virus（PRV） ， two pairs of special primers were
designed， duplex PCR for distinguishing the wild strain and vaccine strain of PRV was established. By this duplex PCR
assay， two specific fragments of 122 bp（gE）and 246 bp（gB）were simultaneously amplified from field isolate
DNA， only a 246 bp（gB）fragment was amplified from vaccine strain DNA. The results indicated that the method was
specific， sensitive， and could be used for rapid diagnosis and epidemiological investigation of PRV infection. |
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