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Cloning and expression analysis of ERF transcription factorgene CmERFII-11 cDNA in melon(Cucumis melo L.) |
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Abstract: |
The specific primers were designed according to reported cDNA sequences of CmERFII-11 in melon,
CmERFII-11 gene cDNA was cloned from fruit of Cucumis melo L. by RT-PCR method. CmERFII-11 gene was
analyzed by the tools of bioinformatics, expression pattern was showed by Real-time quantitative PCR. The length
of cDNA was 954 bp coding a polypeptide of 255 amino acids with a calculated molecular weight of 28.33 ku and an
isoelectric point of 7.62. CmERFII-11 gene expressed in the root, stem, leaf and different developmental stages of fruit
after pollination, CmERFII-11 gene expressed highest in leaf. |
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