文章摘要
Cloning and expression analysis of ERF transcription factorgene CmERFII-11 cDNA in melon(Cucumis melo L.)
  
DOI:
Author NameAffiliation
额贺希格, 鲍牧兰, 邵 琪, 柴运佳, 哈斯阿古拉 内蒙古大学生命科学学院 / 内蒙古自治区牧草与特色作物生物技术重点实验室 内蒙古 呼和浩特 010021 
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Abstract:
      The specific primers were designed according to reported cDNA sequences of CmERFII-11 in melon, CmERFII-11 gene cDNA was cloned from fruit of Cucumis melo L. by RT-PCR method. CmERFII-11 gene was analyzed by the tools of bioinformatics, expression pattern was showed by Real-time quantitative PCR. The length of cDNA was 954 bp coding a polypeptide of 255 amino acids with a calculated molecular weight of 28.33 ku and an isoelectric point of 7.62. CmERFII-11 gene expressed in the root, stem, leaf and different developmental stages of fruit after pollination, CmERFII-11 gene expressed highest in leaf.
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