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| DAS-ELISA detection of pepper and tomatoviruses in Hainan province |
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| Abstract: |
| Peppers and tomatoes are important economical agricultural crops，several viruses cause yield losses
of pepper and tomato. In 2013-2014，253 pepper and 289 tomato virus disease samples were collected in Hainan
province，7 main viruses and the relative content of virus were measured by double antibody sandwich enzyme-linked
immunosorbent assay（DAS-ELISA）. The results showed that the overall detection rate of pepper virus disease was
59.68% and CMV was the highest（41.11%）. There were 7 compound infection types and infection rate was 30.83%，
CMV and TMV were the advantage virus sources of pepper. The overall detection rate of tomato virus disease was 44.64%
and TYLCV was the highest（22.84%）. There were 10 compound infection types and infection rate was 15.57%，
TYLCV and CMV were the advantage virus sources of tomato. Determination of the relative content of pepper samples
showed that CMV was the highest and BBWV was the lowest. Determination of the relative content of tomato samples
showed that TYLCV was the highest and BBWV was the lowest. The content of CMV was higher in pepper samples than
that in tomato samples，but the content of other viruses were lower. |
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