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| Determination of adenosines in muscle and intestinaldigesta of Landrace pigs by high preformanceliquid chromatography |
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| Abstract: |
| Adenosines are the vector of energy metabolism. The aim of this work was to establish a method
for the differences of energy metabolism from the pig muscle and intestinal digesta by high preformance liquid
chromatography（HPLC）.When the analytic condition of HPLC in feed was a reverse phase Waters C18 column
（250 mm×4.6 mm，5 μm），mobile phase was methanol（V）/ 50mmol/L KH2PO4 buffer solution（pH6.5）
（V 9∶91） with the flow rate of 0.5 mL/min，the column temperature was at 30（±1）℃，the detection
wavelength was operated at 259 nm，and the injection volume was 10 μL，the calibration curves had good
linearity over concentration range of 0.1-100 mg/L. The linear relative coefficient for ATP，ADP and AMP over
concentration range of 0.1-100 mg/L were 0.999，0.998 and 0.996，respectively. The limit of detections（LOD，
S/N ≥ 3）of ATP，ADP and AMP were 0.0001，0.004 and 0.004 mg/L，and their limit of quantifications
（LOQ，S/N ≥ 10）were 0.002，0.02 and 0.02 mg/L，respectively. The mean recovery ratio of ATP，ADP
and AMP were 92.00%-99.05%，100.77%-104.48% and 110.84%-113.72% with RSDs of 0.29%-1.02%，
0.33%-1.65% and 0.69%-1.92%，respectively. The whole time of analysis lasted for 10 min.This method has
properties of rapidity，accuracy，sensitivity and
repeatability，which can be applied in detecting the
concentration of ATP，ADP and AMP and analyzing
the energy metabolism. |
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