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| Establishment and application of a method for measurementof rice grain shape based on image analysis |
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| Abstract: |
| In this study，a new method with accurate，efficient，high-throughput measurement of rice grain
shape was established based on image analysis. Digital images of rice grain samples were fetched batching using
scanner，segmented preprocessing with image cropping tool and analyzed by SmartGrain software to get grain shaperelated
metrics. Using this method，we could measure a large number of different samples of grain length，width，area
and roundness and other traits simply and rapidly. The same samples were measured by manual scale measurement
method and SmartGrain software，and their results were used to do significance test. The results showed that no
significant difference of measurement result in the two kinds of measurement methods，with correlation coefficient
value nearly equaling to 1，meaning very strong correlation. The new measurement method for rice grain shape was
applied to a recombinant inbred line population in high-throughput determination of multiple grain shape traits，to
improve efficiency of measurement. |
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