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| Research on germplasm in vitro preservation technologyof endangered plant Begonia fimbristipula |
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| Abstract: |
| Taking tube seedlings of endangered plant Begonia fimbristipula as materials，the best way of in vitro
preservation of germplasm was studied. The results showed that conventional medium 6-BA and NAA combination
was not suitable for germplasm in vitro preservation of B. fimbristipula，the survival rate was only 5% after 90 d. PP333
inhibited the differentiation of tube seedlings sprout plexus，significantly promoted the formation of adventitious roots，
after 180 d，only the survival rate of 3.0 mg/L PP333 treatment reached 55%，variation of germplasm in restoring growth.
ABA（0.5-2.0 mg/L）inhibited the growth of tube seedlings，after 180 d，the test-tube seedling survival rate was
100%，the somaclone was better after recovery training. 1/2 MS culture medium had the best effect on B. fimbristipula，
the survival rate was as high as 85% after 240 d. After restoring growth，the tube seedlings multiplication and their
growth were superior to other processings. 1/2 MS culture medium can be used as the best training method for germplasm
in vitro preservation of B. fimbristipula. |
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