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| Study on dual channel molecularly imprinted sensorfor chloramphenicol detection in milk |
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| Abstract: |
| Taking chloramphenicol（CAP）as template molecule，and o-phenylenediamine（o-PD）as
functional monomer，poly（o-phenylenediamine）（PPD）film was electropolymerized on the surface of a glassy
carbon electrode（CAP-PPD/GCE）and a dual channel screen-printed carbon electrode（CAP-PPD/SPCE）
using cyclic voltammetry（CV），respectively. Furthermore，CAP molecularly imprinted electrode（CAP-MIP/GCE
and CAP-MIP/SPCE）was fabricated by eluting the template molecules using the mixed solution of acetic acid and
alcohol. CAP was indirectly determined based on potassium ferricyanide as electroactive probe．The properties of
molecularly imprinted sensor were investigated. The response current of the two sensor had good linear relationship
with the concentration of CAP in the range of 1.0×10-5-1.0×10-4 mol/L and 2.0×10-5-1.4×10-4 mol/L. The
detection limit was 5.2×10-6 mol/L and 1.9×10-6 mol/L，respectively. The proposed method was applied to detect
CAP in commercial pure milk，and ultraviolet spectrophotometry was used to verify. The results showed that the
proposed sensor had the advantages of good selectivity
and high accuracy，and was suitable for determination
of CAP in milk samples. Compared to CAP-MIP/GCE，
the dual channel sensor could achieve two repetitive
measurements by using a small amount of sample（50
μL），furthermore，test efficiency and reproducibility
were obviously improved |
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