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| Cloning and bioinformatics analysis ofcatalase gene in upland rice |
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| Abstract: |
| In order to study the sequence characteristics and biological information of CAT gene in upland rice，
the CAT gene of upland rice of Luhan No.3 was cloned by homology cloning methord（OsCATB-lh，GenBank accession
number：KR133179）. The cDNA length of OsCATB-lh was 1 739 bp，with an open reading frame of 1 479 bp encoding
a polypeptide of 492 amino acids. OsCATB-lh was a single copy gene in rice genome onthe sixth chromosome . The
protein encoded by OsCATB-lh dcontained a catalase family conserved domain. The high homology of cDNA（99%）
with 6 single nucleotide polymorphisms（SNP）sites and protein（99%）with 3 amino acid mutation sites were found
between OsCATB and OsCATB-lh. |
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