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| Detection of main viral species of vegetablevirus deseases in Guizhou |
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| Abstract: |
| DAS-ELISA method was used to detect 952 suspected vegetable virus samples which were planted in
diverse planting regions in Guizhou Province，6 virus DAS-ELISA tests：CMV，TMV，TuMV，TSWV，BBWV and
CGMMV were conducted. The results showed that the positive samples number were 273，which accounted for 28.68%
of the total samples. CMV took the highest positive rate with 16.49%，followed by other 5 viruses，orderly with 2.63%，
1.58%，0.53%，1.05% and 2.10%，respectively. It suggested that the major virus type infecting vegetables was CMV in
Guizhou Province. According to the infection of single virus，CMV was found in all 12 vegetables. TuMV was found in
eggplant，Chinese cabbage，radish and cabbage，and TSWV in pepper and tomato. There were 6 complex virus infection
types，including CMV+TMV，CMV+TuMV，CMV+TSWV，CMV+BBWV，TuMV+BBWV and CMV+TuMV+BBWV.
The positive rate of CMV+TMV was 2.52%，higher than others. In the vegetable planting regions in city suburbs，the
vegetables of solanaceae leguminosae were infected the most，the vegetables of cruciferae were infected the least，the
vegetables of Cucurbitaceae were more in the thermophilic vegetable planting regions in winter and spring. The results
show that it is suitable for virus growth in the high altitude temperature areas than in the low altitude warm valley areas. |
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